Effects of 1-MT on number of IFN-γ-secreting human CD8⁺ cells and HIV-1-specific CD8⁺ precursors. Number of IFN-γ-secreting cells in the peripheral blood one week after HIV-1 MDM injection (A,B). Representative histograms of human CD8/IFN-γ cells in control and 1-MT-treated groups are shown in panel A. Average percentage of IFN-γ-secreting cells (n = 7 and n = 8 for control and 1-MT mice, respectively) in peripheral blood (1-3 weeks) (B). Percentage of HIV-1^gag (SL-9) tetramer-positive human CD8⁺ cells from spleen expanded by PHA/IL-2 stimulated in vitro 3 weeks after HIV-1 MDM injection (C). Two left panels are control animals, 2 right panels are 1-MT-treated mice. Error bars indicate SEM. ^*P < .05 (1-MT versus vehicle control).