Localization of IgG and C components on mesenteric tissue of rats treated with LPS followed 3 hours later by aPL-positive IgG. The mesenteric tissue was double-stained for IgG (A,C) using FITC-labeled goat anti-human IgG and for C3 (B) or C9 (D) using biotin-labeled specific antibodies to rat C components (5 μg/mL) followed by RPE-conjugated streptavidin. Original magnification, × 250. Details of image acquisition are given in Dobrina et al.²³