Figure 2.
Immunoblot analysis of HIF-1α protein expression in conventional, oxygen-impermeable culture dishes versus gas-permeable dishes. (A) Human osteosarcoma cells (U2OS) were exposed to intermediate hypoxia (3% O2) for 18 hours on the indicated type of culture dish. (B) Human osteosarcoma cells (143B-wt) and their mitochondrial DNA-deficient derivative 143B-206ρ0 were grown on conventional or gas-permeable cell culture dishes and incubated in 20% O2, 1% O2, or 3% O2. Confluent 143B-wt expresses detectable levels of HIF-1α. (C) Comparison of 143B-wt and 143B-206ρ0 cells cultured on conventional versus oxygen-permeable supports at 3% oxygen.
