Figure 4.
HIF-1α protein expression in U2OS cells exposed to inhibitors of the electron transport chain in combination with PHD inhibitors. Cells were exposed to DMOG (100 μM) in normoxia, or to antimycin A (1 μg/mL) or rotenone (1 μM) in normoxia (NOX) or in hypoxia (3% O2, HOX) for 4 hours. Additionally, a combination of an ETC inhibitor and DMOG was applied to the cells.
