Effect of HHV-8 on HIV in primary macrophages. Primary MDMs from healthy donors were infected with HHV-8 or transfected with pCR-50sp and were subsequently infected with monotropic strains HIV_BaL (A,C) and HIV_ADA (B,D) at a 1:1000 MOI; p24 antigen release (A-B) and HIV particles production (C-D) were evaluated. Values represent the mean of duplicate samples of 2 separate experiments. Error bars indicate SD. (E) The results are shown of rtPCR amplification of the indicated HHV-8 genes using 200 ng total RNA extracted from infected cells; β-actin amplification is shown as a control. Control for DNA contamination, performed by direct β-actin amplification of RNA without retrotranscription, is also shown.