Characteristics of B-cell depletion and kinetics of B-cell recovery. Flow cytometric analysis of PB, spleen, BM, and LN before and 12 days after 2 injections with CD22/cal demonstrating that CD22/cal is not cytotoxic for (A) CD3⁺ T cells and (B) Gr-1⁺ myeloid cells. (C) Flow cytometric analysis showing the kinetics of CD22⁺ B-cell recovery in BM samples. (D) Flow cytometric analysis demonstrating that there is complete recovery of CD22⁺ B cells in PB, spleen, BM, and LNs 50 days after the first injection with CD22/cal. Three mice per group were analyzed; representative data from one mouse are shown.