Peripheral B-cell maturation in Vav-deficient mice. Flow cytometric analysis of splenocytes gated for lymphocytes by forward and side scatter. (A) Cells from the indicated genotype were stained for CD93, CD23, and IgM. CD93⁺ cells were further analyzed by the expression of IgM and CD23. Gates indicate T1 (IgM^high/CD23^low), T2 (IgM^high/CD23^high), and T3 (IgM^low/CD23^high) B cells. Numbers indicate the percentage of cells falling into each gate. CD93^- cells were further subdivided into FO mature (CD23^high, IgM⁺). Percentages of cells falling in the mature gate are shown. (B) Cells from the indicated genotypes were stained with anti-CD21 and anti-CD23 antibodies. Percentages refer to cells in the MZ B-cell gate (CD21^high/CD23^low). (C) Absolute numbers of T1, T2, T3, mature FO, and MZ (defined as CD21^high, CD23^low) cells. Results are presented as mean ± SD, respectively, from wild-type (10 mice; □), Vav-1/Vav-2 (10 mice; ▧), and Vav-deficient mice (4 mice; ▪). Significant differences between wild-type and Vav-1/Vav-2 mutant mice or wild-type and Vav-deficient mice are indicated. ^*P < .02; ^**P < .0001. Statistical analysis was performed using the Student t test.