Bcl-2 and A1 levels are reduced in Vav-deficient B cells. (A) (▪) Levels of A1, Bcl-2, Mcl-1, and CXCR5 mRNA in Vav-deficient mature B cells. (□) Wild-type levels. (B) Protein levels expressed by B-cell subsets. Spleen cells from wild-type or Vav-deficient mice were stained for Bcl-2, Bcl-XL, or CXCR5 in combination with anti-CD93, anti-CD23, and anti-IgM. Bar graphs show the mean fluorescence intensity (MFI) (± SD) of Bcl-2, Bcl-XL, and CXCR5 levels from the splenic B-cell subsets for 4 mice of each genotype. Protein levels were calculated subtracting the MFI of the isotype control from the MFI of the protein of interest. Protein expression in the spleen is represented as follows: T1 (open columns); T2 (stippled columns); T3 (gray columns); mature FO (hatched columns). Significant differences between wild-type and Vav-deficient mice are indicated by asterisks (^*P < .01). Statistical analysis was performed using the Student t test.