PP2, wortmannin, and Ro31-8220 inhibited aggregation, TxA2 production, and ATP secretion; PP2 inhibited Akt, Syk, and PLCγ2 phosphorylation; and wortmannin inhibited Akt and PLCγ2, but not Syk phosphorylation. (A) Aggregation and agglutination traces of wild-type platelets treated with bt/VWF in the presence of 10 μM PP2, 100 nM wortmannin, and 10 μM Ro31-8220. All of these inhibitors eliminated the aggregation, but did not affect agglutination. (B) PP2, wortmannin, and Ro31-8220 prevented TxA2 production and ATP secretion. (C) PP2 and wortmannin inhibited the phosphorylation of Akt. (D) PP2 inhibited the Syk and PLCγ2 phosphorylation. Wortmannin inhibited phosphorylation of PLCγ2, but not Syk. The error bars represent standard deviation; n = 3. In some cases, the values of the SD were so small that the bars cannot be seen.