Figure 4.
In vivo engraftment capacity of CD14+/VEGFR-2+ and CD14+/VEGFR-2- cells. Immunohistochemical staining of sections from balloon-injured mouse femoral artery with an anti-GFP antibody showed (A) no localization of GFP+ areas in sham-transplanted and (B) CD14+/VEGFR-2--transplanted cells on the luminal surface of the seeded artery. (C) Staining with control secondary antibody. (D) Significant number of GFP+ (red) CD14+/VEGFR-2+ cells (arrows) at 2 weeks and (E-F) 4 weeks were found in the animals that received these cells. Original magnification, 60 ×.