Immunofluorescence staining for detection of CD14⁺/VEGFR-2⁺ and CD14⁺/VEGFR-2^- cells after transplantation. Human nucleus antibody stained cells (red) in normal human artery (A) but not in control-injured, sham-transplanted mouse artery (B). (C) Balloon-injured mouse femoral artery showed the localization of transplanted human CD14⁺/VEGFR-2⁺ cells on the luminal surface of the seeded artery (red cells, arrows) at 4 weeks. (D) No localization of transplanted CD14⁺/VEGFR-2^- cells was observed at 4 weeks. (E) The endothelial cell phenotype of transplanted human cells was confirmed by double staining with human nucleus antibody and antibodies to VE-cadherin (double-positive cells are stained yellow; arrows). Original magnification, 60 ×.