Figure 1.
Transcription factor-binding motifs in the FCAR promoter and oligonucleotides used for EMSA. Binding sequences for Ets and C/EBP family members (CE1, CE2, and CE3) in the regulatory region of the FCAR gene are indicated by arrows. The numbers indicate nucleotide positions relative to the translation initiation ATG at +1. Wild-type oligonucleotides between -103 and -74 (wtETS) and between -98 and -79 (wtETS-s) used in EMSA contain an Ets-binding site. Specific base pair substitutions used to mutate the Ets-binding site in mutant oligonucleotides (mutETS and mutETS-s) are indicated in bold.