The FCAR promoter is activated by GABP. (A) A schematic representation for the structure of the wild-type and mutant FCAR upstream-luciferase (Luc) constructs. X indicates Ets site mutation. (B) HeLa cells were transfected with 0.7 μg luciferase reporter constructs containing the indicated FCAR promoter in the absence or presence of 0.1 μg each of expression vector(s) for GABPα and/or GABPβ, along with 5 ng pRL-CMV; each sample was transfected with the additional corresponding empty vector pCR3.1E to bring the amount of transfected DNA in each sample to 1 μg. Firefly and Renilla luciferase activities were determined 24 hours after transfection. Values are corrected for transfection efficiency and presented as the fold stimulation of each construct by GABPα and/or GABPβ, compared with the promoter activity seen without the expression vector(s). Thin bars represent the SE from 3 transfections.