American Society of Hematology

Figure 1.

$Figure 1. Purification of the Hx-heme receptor from placenta and liver by Hx-heme affinity chromatography. (A) SDS-PAGE of the purified human Hx used for coupling to heme and the Sepharose matrix. (B) SDS-PAGE showing the elution profile of the eluate from the Hx-heme-Sepharose column loaded with solubilized human placenta membranes. (C) SDS-PAGE of the eluate (lane 1, peak fraction) from a Hx-heme-Sepharose column loaded with human liver membranes. Lanes 2 and 3 show Western blotting of liver Hx-heme affinity eluate using the monoclonal antibodies A2MRα-2 and A2MRβ-1, which recognize the LRP/CD91 α- and β-subunits, respectively. Lane 4 shows SDS-PAGE of the eluate from a Hx-heme column after multiple (> 10) loading-elution runs. Notice that the asialoglycoprotein receptor (ASGP-R) now is becoming a predominant band.$

Purification of the Hx-heme receptor from placenta and liver by Hx-heme affinity chromatography. (A) SDS-PAGE of the purified human Hx used for coupling to heme and the Sepharose matrix. (B) SDS-PAGE showing the elution profile of the eluate from the Hx-heme-Sepharose column loaded with solubilized human placenta membranes. (C) SDS-PAGE of the eluate (lane 1, peak fraction) from a Hx-heme-Sepharose column loaded with human liver membranes. Lanes 2 and 3 show Western blotting of liver Hx-heme affinity eluate using the monoclonal antibodies A2MRα-2 and A2MRβ-1, which recognize the LRP/CD91 α- and β-subunits, respectively. Lane 4 shows SDS-PAGE of the eluate from a Hx-heme column after multiple (> 10) loading-elution runs. Notice that the asialoglycoprotein receptor (ASGP-R) now is becoming a predominant band.

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