Low-avidity T cells are less susceptible to GAD TMr-induced apoptosis. (A-D) High-avidity (A,C) and low-avidity (B,D) T cells were cultured for 12 hours in the presence of 10 μg/mL GAD TMr (black profiles), 4 μg/mL anti-CD3 mAb (gray profiles), or control TMr (dashed profiles). Ann-V staining profiles are shown and the percent increase in Ann-V⁺ cells indicated. (E) Percent increase (mean ± SE of 2 independent experiments) in Ann-V⁺ cells for high- and low-avidity T cells treated at different GAD TMr concentrations.