American Society of Hematology

Figure 4.

$Figure 4. Apoptosis-resistant high- and low-avidity T cells become similarly anergic but acquire differential suppressive properties on GAD TMr treatment. Following 24 hours of treatment with control or GAD TMrs and resting for 72 hours, viable (Ann-V-) T cells were sorted and rechallenged on GAD-pulsed B cells (1 × 104/well), in the absence (A) or presence (B) of IL-2 (10 U/mL). Control and GAD TMr-treated fractions from high- and low-avidity T cells were cultured either separately (3 × 104/well; □ and ▪, respectively) or mixed together (3 × 104 each/well; ▧). 3H-TdR incorporation after 60 hours of culture is shown. P for the comparison between control TMr-treated and mix conditions, as calculated by 2-tailed Student t test, is indicated in panel A. Data are mean ± SE.$

Apoptosis-resistant high- and low-avidity T cells become similarly anergic but acquire differential suppressive properties on GAD TMr treatment. Following 24 hours of treatment with control or GAD TMrs and resting for 72 hours, viable (Ann-V^-) T cells were sorted and rechallenged on GAD-pulsed B cells (1 × 10⁴/well), in the absence (A) or presence (B) of IL-2 (10 U/mL). Control and GAD TMr-treated fractions from high- and low-avidity T cells were cultured either separately (3 × 10⁴/well; □ and ▪, respectively) or mixed together (3 × 10⁴ each/well; ▧). ³H-TdR incorporation after 60 hours of culture is shown. P for the comparison between control TMr-treated and mix conditions, as calculated by 2-tailed Student t test, is indicated in panel A. Data are mean ± SE.

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