American Society of Hematology

Figure 3.

$Figure 3. β1-tubulin protein expression. (A) Immunoblot analysis of β1-tubulin, β-tubulin, and Gq alpha in the total platelet protein fractions (10 or 50 μg) from healthy controls and 4 to 6 unrelated heterozygous Q43P carriers. Gq alpha serves as a loading control. (B) Contrast microscopy of GPF-tagged β1-tubulin in MEG01 megakaryocytic cells transfected with pEGFP-WT-β1-tubulin or pEGFP-Q43P-β1-tubulin (top 4 rows). Immunofluorescence analysis of transfected MEG01 cells on Horm collagen (bottom left panels) or fibrinogen (bottom right panels). Original magnification, × 20.$

β1-tubulin protein expression. (A) Immunoblot analysis of β1-tubulin, β-tubulin, and Gq alpha in the total platelet protein fractions (10 or 50 μg) from healthy controls and 4 to 6 unrelated heterozygous Q43P carriers. Gq alpha serves as a loading control. (B) Contrast microscopy of GPF-tagged β1-tubulin in MEG01 megakaryocytic cells transfected with pEGFP-WT-β1-tubulin or pEGFP-Q43P-β1-tubulin (top 4 rows). Immunofluorescence analysis of transfected MEG01 cells on Horm collagen (bottom left panels) or fibrinogen (bottom right panels). Original magnification, × 20.

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