Reduced ferroportin expression in the spleen of IRP2-deficient mice. (A) Ferroportin expression was analyzed by fluorescent immunostaining on spleen sections. The red pulp (RP) and the white pulp (WP) are indicated. Macrophages of the red pulp were revealed with an anti-F4/80 antibody. Nuclei were stained with Dapi. Pictures were acquired using a wide field Axiovert 200M microscope and a × 25 immersion objective from Zeiss. (B) Ferroportin expression was analyzed in groups of 4 +/+, +/-, and -/- mice by Western (top panels) and Northern (bottom panels) blotting. The histogram depicts the levels of ferroportin expression after normalization for β-actin. Error bars indicate standard deviation. These figures are representative of data obtained with 3 independent lots of mice (including 4 +/+ and 4 -/- animals each).