The lipid phosphatase activity was essential for PTEN's role as a negative regulator of chemotaxis. Tet-on Jurkat cells were transiently transfected with Tet-inducible vectors expressing PTEN/wt, PTEN/G129E, PTEN/G129R, or a mock vector. After 6 hours, dox (1 μg/mL) was added to the culture medium. Cells were cultured for an additional 48 hours and then harvested for Western blotting (A) and chemotaxis (B). The results were representative of 3 independent experiments. Data shown are means ± SD.