Suppression of hemojuvelin mRNA results in the suppression of hepcidin mRNA. Each individual point represents an experiment in which Hep3B cells were treated with one of the siRNA preparations overnight and then incubated for an additional 24 hours before mRNA extraction. In each experiment, hemojuvelin and hepcidin mRNA were quantified by qRT-PCR and normalized to the housekeeping gene G3PD. Control cells were treated only with transfection reagents and their hemojuvelin/G3PD and hepcidin/G3PD ratios were set as baseline at 1. (A) Closed symbols represent hemojuvelin siRNAs experiments (▪ indicates HJVsi1; •, HJVsi2; ▾, HJVsi3; ▴, HJVsi4). The regression line (all HJV siRNAs experiments, R = 0.64, with 95% confidence limit) indicates that as the HJV/G3PD ratio decreases, there is a corresponding decrease in the hepcidin mRNA/G3PD ratio. (B) Open symbols represent siRNA control experiments (▵ indicates NCsi1; ○, NCsi2; □, NCsi3). No consistent effect on hepcidin is seen with control siRNAs. Note the larger horizontal scale compared to panel A.