sFRP-2 immunodepletion restores suppression of TCF reporter activity in osteoblasts by MM cell CM. Osteobalsts were cotransfected with TCF reporter plasmid and pRL-TK Renilla luciferase plasmids as described in “Patients, materials, and methods.” The transfected osteoblasts were then treated with RPMI8226 CM with or without sFRP-2 immunodepletion at 20% in the presence of 50 ng/mL rhBMP-2. Data were corrected for Renilla luciferase and are expressed as a mean of 6 independent experiments with an error bar of SD in arbitrary units. * indicates significantly different by Student t test, P < .05.