The panel of rupture force spectra demonstrating the interactions of desA-fibrin and desA-NDSK with fibrinogen and fragment D along with control data for nonspecific protein-protein binding. (A,D,G) Pure interactions between desA-fibrin and fibrinogen, desA-NDSK and fibrinogen, desA-NDSK and fragment D, respectively; (B,E,H) the same interactions in the presence of 1 mM GPRPam; (C,F,I,J) negative controls with one or both interacting surfaces coated with the proteins lacking complementary D/E binding sites: fibrinogen versus fibrinogen (C), NDSK versus fibrinogen (F), desA-NDSK versus BSA (I), fibrinogen versus BSA (J). Error bars indicate standard deviation.