American Society of Hematology

Figure 1.

$Figure 1. Expression of the scFv-CD28-ζ receptor in transduced CD8+ and CD4+ primary mouse T cells. Splenic T cells enriched from BALB/c mice were depleted into CD4+ and CD8+ T-cell subsets or left as unfractionated T cells prior to retroviral transduction with the scFv-CD28-ζ receptor. Transduced unfractionated T cells consisted of 80% to 85% CD8+ T cells and about 10% CD4+ T cells (A), whereas isolated populations consisted of more than 90% CD8+ (C,G) or CD4+ T cells (E,I). Chimeric scFv receptor expression was detected in unfractionated transduced T cells (B), CD8+ (D), and CD4+ (F) T cells by flow cytometry following staining with an anti-tag mAb and PE-labeled sheep anti–mouse immunoglobulin (solid line) or with the PE-labeled secondary alone (dashed line). Negligible receptor expression was detected in isolated populations of CD8+ (H) and CD4+ (J) T cells transduced with the empty LXSN vector. Similar results were obtained in 10 experiments.$

Expression of the scFv-CD28-ζ receptor in transduced CD8⁺ and CD4⁺ primary mouse T cells. Splenic T cells enriched from BALB/c mice were depleted into CD4⁺ and CD8⁺ T-cell subsets or left as unfractionated T cells prior to retroviral transduction with the scFv-CD28-ζ receptor. Transduced unfractionated T cells consisted of 80% to 85% CD8⁺ T cells and about 10% CD4⁺ T cells (A), whereas isolated populations consisted of more than 90% CD8⁺ (C,G) or CD4⁺ T cells (E,I). Chimeric scFv receptor expression was detected in unfractionated transduced T cells (B), CD8⁺ (D), and CD4⁺ (F) T cells by flow cytometry following staining with an anti-tag mAb and PE-labeled sheep anti–mouse immunoglobulin (solid line) or with the PE-labeled secondary alone (dashed line). Negligible receptor expression was detected in isolated populations of CD8⁺ (H) and CD4⁺ (J) T cells transduced with the empty LXSN vector. Similar results were obtained in 10 experiments.

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