Delineation of a common interval of deletion of 0.6 Mb in 8p21.3 in B-cell lymphomas. (A) Array CGH analysis shows deleted clones (red), genomic gains (green), nondeleted probes (white), and noninformative clones (orange). Asterisks indicate the clones used for verifying array CGH results by FISH analysis in selected cell lines. (B) Array CGH analysis of chromosome 8 in PR1 and Z138 cell lines, and in patient P005, showing genomic deletions in 8p21 undetected using conventional CGH. (C) G-banding cytogenetic, FISH, and SKY (spectral karyotyping) analyses show deletion of chromosome 8p and translocation of the remaining nondeleted allele in OCI-Ly8 cell line in a complex translocation. (D) FISH analysis of 8p21.3 deletion using BAC RP11-89M8 (green) and a centromeric chromosome 8 probe (red). The examples correspond to 2 patients with transformed splenic marginal zone lymphoma (left) and DLBCL (right). (E) Map of chromosome 8p21.3 including the clones used for narrowing down the commonly deleted interval by array CGH, SMRT array, and FISH (black) (UCSC, May 2004 freeze).53 Inset shows the SMRT array CGH analysis of 2 selected cell lines and one primary tumor.
