Gene expression analysis of tumors with 8p deletion. (A) Representation of gene expression analysis of cell lines with deletion of 8p (left) and those without genomic deletion (right). Array indicates the position of TRAIL receptor genes in the array. The 5 clones with statistically significant decreased expression in cell lines with deletion versus nondeleted strains are shown. (B) RT-PCR analysis for TRAIL receptor genes and the caspase 8 gene. (C) Flow cytometry analysis for TRAIL receptor genes showed reduced expression for TRAIL-R1 and TRAIL-R2 in deleted versus nondeleted cell lines and patient samples. (D) Methylation analysis: representative examples of cell line samples. U indicates unmethylated; M, methylated; IVD, methylated positive control. In red, cell lines showing methylation of the corresponding gene.