Development and characterization of DC biosensor system. (A) XS106 DCs were incubated for 6 hours with the indicated agents, including necrotic keratinocyte preparations (Nec KC), and then examined for cytokine mRNA profiles by RNase protection assay. (B) The XS106-pIL1-YFP DC biosensor clone was incubated for 16 hours with LPS at the indicated concentrations and then examined for the mean fluorescence intensity (MFI) for YFP signals (mean ± SD from triplicate samples). (C) The same clone was incubated with 30 ng/mL LPS (•) or PBS alone (○) for the indicated periods and then examined for YFP expression (mean ± SD; n = 3). Asterisks indicate statistically significant (**P < .01) differences compared with the nontreated control samples. Data shown in this figure are representative of at least 3 independent experiments producing similar results.