Quantification of hematopoietic and myeloid-related transcripts in fractions A1 to A3. □ indicates A1; ▦, A2; ▨, A3, and ▪, adult F4/80⁺ peritoneal Mφ (taken as a control). (A) The histogram shows a dramatic decrease from A1 to A3 in the expression of the AML-1, GATA-2, LMO-2, and TAL-1 transcription factors involved in the transition from mesoderm to hematopoiesis. (B) Quantification of lysozyme M and MPO transcripts. The histogram shows a gradual increase in the transcripts for lysozyme M from A1 to A3, but only transient expression of MPO in fractions A1 and A2. These variations correlated with those observed during adult macrophage maturation.