Abnormal proportions of splenic DC subsets in Jak3^-/- mice. (A) DC-enriched low-density cells from wild-type (Wt) and Jak3^-/- spleens were stained with anti-CD4, anti-CD8, anti-B220, anti-Gr1, and anti-CD11c. The proportion of CD11c⁺ cells is presented in the left column. Gating on CD11c⁺ cells, the proportions of different DC subsets are illustrated in the middle and right columns. (B) The absolute number (mean ± SD) of each DC subset in wild-type and Jak3^-/- spleens from 8 separate experiments is shown. ^*P < .05 and ^**P < .01 compared with wild type (n = 8). (C) Activated phenotype of Jak3^-/- splenic DCs. DC-enriched low-density cells from wild-type (broken line) and Jak3^-/- (solid line) spleens were gated on CD11c⁺ and analyzed for MHC class II, CD40, CD80, and CD86 expression. Membrane expression of these proteins was assessed by flow cytometry and is compared with staining with an isotype control antibody (shaded histograms). Data are representative of 5 separate experiments with similar results.