Enhanced survival of Jak3^-/- and γc^-/- BMDCs. (A-B) Purified BMDCs from wild-type, Jak3^-/-, γc^-/-, and IL-15^-/- mice were cultured in medium lacking cytokines and stained with APC-conjugated annexin V and propidium iodide at the indicated time points. Cells that were negative for staining of both annexin V or PI were designated as live cells. These data are representative of more than 5 separate experiments. (C) Bak and Bax mRNA expression. Purified BMDCs were cultured in medium lacking cytokines for 12 hours and RNA was isolated. Bak and Bax levels were determined by RNase protection assay. L32 probes were used to normalize sample load in each well. The data are representative of 3 separate experiments.