Suppression of SAP expression by LMP1 in T cell lines. (A) Protein expression of SAP in 2 T-cell lines (H9 and Jurkat) and 1 B-cell line (BJAB) transfected with pSG5-LMP1 constructs. SAP protein could be detected on both H9 and Jurkat cells, but not on BJAB cells. The expression of SAP was strongly reduced on LMP1-H9 cells and mildly decreased on LMP1-Jurkat cells. Cells transfected with the pSG5 construct were used as vector control groups. The quantitative results of SAP expression adjusted with α-actin are shown below the blots. Error bars representing the standard deviation of 3 independent experiments are shown. (B) The mRNA expression of SAP in H9 cells. SAP gene transcripts on H9 and LMP1-H9 cells were detected by RT-PCR, and products collected at different PCR cycles were resolved on the agarose gels for quantitation.