Figure 1.
Colonies formed under various conditions. Top and bottom panels show the appearance of colonies under an inverted microscope (ITM; Olympus, Tokyo, Japan) equipped with a camera module (DP50; Olympus) (objective lens, SPlan 4PL; numerical aperture, 0.13; magnification, × 4) and their constituent cells stained with May-Grünwald-Giemsa solution (microscope, BX51, Olympus; camera module, XC-003, Sony, Tokyo, Japan; objective lens, UPlan F1, Olympus; numerical aperture, 1.3; magnification, × 100), respectively. (A) A spontaneous GM colony in the culture of JMML BM cells without hematopoietic factors. (B) A GM colony in the culture of normal BM cells with 10 ng/mL GM-CSF. (C) A spontaneous G colony in the culture of JMML BM cells with 10 μM ZOL.