TCR-transduced T cells from healthy and CML donors can kill purified CD34⁺ cells of CML and AML patients. (A) The cytotoxicity of TCR-transduced CTLs of a healthy donor was tested against freshly isolated CD34⁺ cells of A2⁺ CML patients (CML1-3), an A2^– CML4 control patient, and against fresh CD34⁺ cells isolated from an A2⁺ healthy donor (N-CD34). (B) TCR-transduced CTLs of a healthy donor were tested against CD34⁺ and CD34^– cells purified of the A2⁺ patients CML5 and AML1. Filled symbols represent CD34⁺ cells and open symbols represent CD34^–. (C) TCR-transduced CTLs of a CML patient were tested against CD34⁺ (•) and CD34^– ◪) cells isolated from the A2⁺ AML2 patient, CD34⁺ cells from the A2⁺ CML5 patient, and fresh CD34⁺ cells from an A2⁺ healthy donor (N-CD34).