Mac-1 is critical to macrophage migration in vivo to the lymphatics. The GFP⁺ WT peritoneal macrophages were injected intraperitoneally into the TG-preconditioned WT mice with and without NIF, followed with intraperitoneal injections of PBS or LPS. (A) The number of GFP⁺ cells within the peritoneum (left) or the draining lymph nodes (right) was determined 4 hours later. The data represent the mean ± SD of 2 independent experiments. (B-D) PKH26-labeled Mac-1^-/- peritoneal macrophages were injected intraperitoneally into the TG-preconditioned Mac-1^-/- mice, followed by intraperitoneal injections of PBS or LPS. The number of fluorescent Mac-1^-/- macrophages within the peritoneum (B), adherent on the peritoneal membrane (C), or within the blood circulation (D) was determined 4 hours later. The data represent the mean ± SD of 2 independent experiments. ^**P < .01; ^*P < .05.