Mitogenic stimulation of Shp2 phosphorylation and relocation. Normal resting hematopoietic cells were stimulated with PHA and collected at different time points for analysis of Shp2 protein levels by Western blotting. The histogram shows p-Shp2 (▦) versus Shp2 (□) contents in samples enriched with resting or proliferating hematopoietic cells. Data are representative of 3 independent experiments.