CCR5 deficiency does not affect the proportions of T_regs in the periphery, the suppressive function of T_regs, FoxP3 expression, proliferation, or survival. (A) CD4⁺CD25⁺ T cells in spleens, and inguinal (ILNs) and mesenteric lymph nodes (MLNs) from male WT and CCR5^-/- mice. Shown are cells within the live lymphocyte gate. Data are representative of 2 organs per group. (B) Suppression of WT responder cell proliferation in response to B6D2 alloantigen by WT (▪) and CCR5^-/- (▨) T_regs was assessed as described in “Materials and methods.” Data represent mean ± SEM. ^*P ≤ .05. (C) FoxP3 expression in WT (▪) and CCR5^-/- (▨) T_regs and CD4⁺CD25^-, cultured as described in “Materials and methods,” was assessed by QPCR. Data represent mean ± SEM.