Online monitoring of intracellular chelation of resident and imposed LIPs. HepG2 cells labeled with CALB via its AM precursor (B, phase contrast and fluorescence) were followed by epifluorescence while perfused in media containing either HBS for up to 1 hour (control cells) or HBS containing the indicated substances added at the indicated times: Fe-HQ (1:1, 10 μM at 22 minutes), DTPA (100 μM at 27 minutes), ICL670 (added at 6-minute intervals from 38-minute time point to give final concentrations of 20, 60, and 100 μM), and SIH (100 μM at 55 minutes). All incubations and perfusions were carried out at 37°C. The montage gallery (A) depicts snapshots of the same field taken during the experiment, and the graph (C) depicts the average fluorescence density of 5 cells in each field (analyzed with NIH Image J [National Institutes of Health, Bethesda, MD]) and normalized to the initial fluorescence intensity (control cells and cells treated with ICL670 at the indicated μM concentration at the indicated time denoted with an arrow). Note: the montage gallery and the single pictures shown in panel B are based on different fields of cells.