Expression of SLAM and SAP in human platelets. (A) Top panel: FACS analysis of resting platelets stained with nonspecific (solid line) or α-SLAM (broken line) IgG-PE and TRAP-activated platelets stained with α-SLAM IgG-PE (dotted line). Bottom panel: platelet activation confirmed by α-CD62P IgG-PE staining of resting (solid line) and activated (dotted line) platelets. (B) RT-PCR of SAP and GAPDH from platelet (lane 1) and spleen (lane 2) cDNA with dH₂O as a control (lane 3). Data are representative of experiments repeated at least 3 to 5 times.