Figure 3.
Figure 3. MLL-AF4 depletion induces apoptosis in t(4;11) cells. (A) Effects of MLL-AF4 suppression on the fraction of sub-G1 cells. Cells obtained from the time courses shown in Figure 2D and E were analyzed for DNA content by flow cytometry. (B) Annexin V staining of SEM cells. Annexin V–positive SEM cells were quantified by flow cytometry 4 days after the second electroporation with 750 nM of the indicated siRNA. The percentages of annexin V and annexin V/propidium iodide–positive cells are given in the corresponding quadrants. (C) MLL-AF4 suppression triggers caspase-3 activation and diminishes BCL-XL protein levels. Immunoblots show BCL-XL and proteolytically activated caspase-3. Tubulin and GAPDH served as loading controls.

MLL-AF4 depletion induces apoptosis in t(4;11) cells. (A) Effects of MLL-AF4 suppression on the fraction of sub-G1 cells. Cells obtained from the time courses shown in Figure 2D and E were analyzed for DNA content by flow cytometry. (B) Annexin V staining of SEM cells. Annexin V–positive SEM cells were quantified by flow cytometry 4 days after the second electroporation with 750 nM of the indicated siRNA. The percentages of annexin V and annexin V/propidium iodide–positive cells are given in the corresponding quadrants. (C) MLL-AF4 suppression triggers caspase-3 activation and diminishes BCL-XL protein levels. Immunoblots show BCL-XL and proteolytically activated caspase-3. Tubulin and GAPDH served as loading controls.

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