Figure 1.
One-dimensional gel separation of CANK cell membrane–enriched fractions. One hundred micrograms of CANK cell membrane–enriched fractions obtained from patient B and his mother were separated on 4% to 20% Tris-glycine gel. Nineteen equally spaced sections between 250 kDa and 6 kDa were excised from each fraction and were subsequently used for proteomic analysis.