Increased mRNA levels of CCR2 receptor in TAP-2–deficient CANK cells. Semiquantative RT-PCR analysis for CCR2 mRNA levels was performed on various cDNA libraries obtained. Healthy donor–derived cDNA was prepared from an mRNA pool purified from equal numbers of CANK cells from 7 healthy persons. cDNA obtained from 1106 melanoma cell line was used as a negative control for CCR2 receptor. RT-PCR analysis was performed to compare intensities of CCR2 levels on CANK cell lines between healthy donors and (A) the mother of patients A, B, and C with TAP-2 deficiency, (B) patient A, (C) patient B, and (D) patient C. Results are from 1 independent experiment of 4 experiments performed.