Specific CTL-mediated lysis of autologous CLL cells. CD8⁺ isolated T cells that were in vitro expanded by 3 rounds of stimulation with autologous MVA-TRICOM–infected CLL cells and exogenous IL-2 were assessed for their ability to lyse autologous, uninfected ¹¹¹In-labeled CLL cells. (A) Four different T-cell cultures established from 4 CLL patients (patients 6, 7, 8, and 9) were assayed. (B) Abs (20 μg/mL) directed against MHC class I or an IgG control were added to ¹¹¹In-labeled target cells for 1 hour before the addition of the T cells (patient 8). Statistical comparison between groups was based on Student 2-tailed t test. Error bars represent the SEM for triplicate determinations.