IL-15Rα⁺ immature lymphoid cells isolated from thymus of a premature infant by reverse laser capture microdissection. The figure shows a nodular pattern of IL-15Rα⁺ lymphoid cells in the thymus before and after rLCM (A-B, with hematoxylin counterstain), and an interstitial pattern of IL-15Rα⁺ lymphoid cells in the thymus before and after rLCM (C-D, phase-contrast view without hematoxylin counterstain). Note the well-preserved histopathology of IL-15Rα⁺ lymphoid cells isolated from a 5-μm frozen section of thymus with use of rLCM. Image acquisition was performed as described for Figure 2.