c-JUN induction by E-R3 depends on JNK and ERK signaling. (A) c-JUN expression was detected by Western blot analysis in mock-infected cells or in cells infected with lentiviral vectors expressing JNK1/2 shRNA. (B) Western blot analysis of c-JUN expression in HUVECs transduced with E-R3 mutant chimeric receptor as indicated at various times after treatment with 10 ng/mL EGF.