c-JUN expression is required for E-R3–dependent survival signal. (A) Western blot analysis of c-JUN from mock-infected cells or cells silenced for c-JUN obtained by transducing HUVECs with a lentiviral vector expressing c-JUN shRNA before or after 2 hours of treatment with 10 ng/mL EGF. (B) Quantification by TUNEL staining of HUVEC apoptosis expressing a mock lentiviral construct or c-JUN shRNA. (C-D) Chemotaxis and mitogenic response of HUVECs either mock infected or silenced by retroviral infection with a vector expressing c-JUN shRNA. The results are expressed as the mean ± SD of 3 independent experiments.