Effect of entry inhibitors on apoptosis and DR5 mRNA expression in T cells. (A) CD4⁺ cell were cultured for 24 hours with AT-2 HIV-1_MN/Ada in the presence of sCD4-IgG (2 μg/mL), AMD-3100 (2 μg/mL), or RANTES (2 μg/mL). Apoptosis was analyzed by flow cytometry using the annexin V method. (B) Titration of sCD4-IgG in blocking AT-2 HIV-1_MN–induced apoptosis of CD4⁺ T cells (μg/mL) (C) Flow cytometric analysis of CXCR4 or CCR5 expression by CD4⁺ T cells cultured for 24 hours in the presence of AT-2 HIV-1_MN with or without AMD-3100 (2 μg/mL) or with or without RANTES (2 μg/mL). (D) DR5 mRNA expression by CD4⁺ T cells cultured in the presence of HIV-1_MN/Ada and with (+) or without (-) sCD4-IgG.