Reconstitution of hematopoiesis by adoptive transfer of Jnk2–/– and Mekk1ΔKDJnk2–/– fetal liver cells. Peripheral blood was drawn every 2 weeks after adoptive transfer of FL cells from Jnk2–/– or Mekk1ΔKDJnk2–/–m E14.5 embryos into lethally irradiated hosts. (A) Samples were analyzed for red blood cell (RBC) quantity, mean corpuscular volume (MCV) of the red blood cells, and white blood cell (WBC) quantity. (B) Peripheral blood cells were drawn 12 weeks after adoptive transfer. Samples were analyzed for cell-surface expression of different lineage markers. The dot plots represent the log fluorescence intensities of live cells (i), or live cells falling into the CD45.2 gate (ii-iv). Monoclonal antibodies used were against Ter119 (erythroid cells), Gr-1 (granulocytes), Mac1 (monocytes), CD4 and CD8 (T lymphocytes), B220 (B lymphocytes), and CD43 (leukocytes). The percentages of cells in each quadrant are indicated.
