1,25(OH)₂D₃ affects ILT3-independent T-cell responses. (A) Monocyte-derived human DCs, incubated for 48 hours with or without 10 nM 1,25(OH)₂D₃, were cocultured with allogeneic CD4⁺ cells for 5 days in the presence or absence of anti-ILT3 mAb or isotype control. Results in panel A refer to mean ± standard error from five separate experiments. (B) CD4⁺ cells were restimulated with anti-CD3 after 2 rounds of restimulation under the same conditions as in panel A. After 72 hours of culture, the indicated cytokines were measured by 2-site ELISA. A representative experiment out of 2 performed is shown in panel B. *P = .002 versus anti-ILT3 without 1,25(OH)₂D₃ by Mann-Whitney U test.