American Society of Hematology

Figure 2.

$Figure 2. Surface phenotype of DCs derived from PBMCs from healthy donors and PBSC harvests from patients with MM. Monocyte fractions were obtained from PBMCs from healthy donors or PBSC harvests from MM patients using a plastic adherence method. These cells were cultured in the presence of IL-4 and GM-CSF for 6 days, followed by stimulation with TNF-α for 24 hours. Cells were stained with either FITC-labeled control mouse IgG (dotted line) or FITC-labeled anti-CD1a, anti-CD40, anti-CD80, anti-CD83, or anti-CD86 MoAbs (solid line) and were analyzed by flow cytometry. The data were representative of DCs from 3 healthy donors and 3 MM patients.$

Surface phenotype of DCs derived from PBMCs from healthy donors and PBSC harvests from patients with MM. Monocyte fractions were obtained from PBMCs from healthy donors or PBSC harvests from MM patients using a plastic adherence method. These cells were cultured in the presence of IL-4 and GM-CSF for 6 days, followed by stimulation with TNF-α for 24 hours. Cells were stained with either FITC-labeled control mouse IgG (dotted line) or FITC-labeled anti-CD1a, anti-CD40, anti-CD80, anti-CD83, or anti-CD86 MoAbs (solid line) and were analyzed by flow cytometry. The data were representative of DCs from 3 healthy donors and 3 MM patients.

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