Staining patterns of 174/2 and PAL-E. (A) Lymph node sections were double stained with an antibody to Lyve-1 (green) recognizing lymphatics in the tissue and 174/2 (red) or PAL-E (red) as indicated in the figure. (B) Vein and aorta specimens were double stained with FITC–anti–factor VIII (FVIII, green) to identify the vascular endothelial cells and 174/2 (red) or PAL-E (red) as indicated in the figure. The insets show stainings with negative control antibodies. Original magnification ×200 (A); ×400 (D).