Cross-precipitations with 174/2 and PAL-E. Lysates of CHO cells transfected with (A) PV-1/FELS cDNA, (B) tonsil, or (C) lymphocyte-depleted tonsil tissue were precleared or not precleared with the indicated antibodies; thereafter, the samples were run on SDS-PAGE and blotted to nitrocellulose membranes. Detection was performed either with 174/2 or PAL-E. neg co indicates negative control. Molecular weight markers are indicated on the left. ME indicates mercaptoethanol.